| abstract |
The invention discloses a method for expressing and preparing a multivalent multispecific antibody and an immune hybrid protein in the technical field of biology; the invention designs and develops a novel method for preparing a multivalent specific antibody, or a hybrid immune protein formed by fusing an antibody and a cytokine, or an immunotoxin formed by fusing an antibody and a toxin protein, or an immune hybrid protein formed by fusing an antibody and other active proteins by utilizing the characteristics of the protein Intein. The present invention expresses each part of the heterozygotic protein in a proper prokaryotic cell system or a proper eukaryotic cell system respectively, and the heterozygotic protein is purified and separated by high-efficiency affinity chromatography and then spliced in vitro under the condition of intein splicing to achieve the purpose of preparing products of the multivalent specific antibody and the immune heterozygotic protein. The method has high production efficiency, is suitable for wide application range, and is favorable for product separation and purification. The present invention provides a novel method for the preparation of biopharmaceuticals for targeted attack on cancer or other diseases. |