http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106370638-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6486 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-31 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2016-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-08-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-08-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106370638-B |
| titleOfInvention | For Hg2+Colorimetric, fluorescence dual signal biosensor and the detection method of detection |
| abstract | The present invention discloses a kind of for Hg 2+ Colorimetric, fluorescence dual signal biosensor and the detection method of detection, including molecule hair fastener probe (H1, H2, H3), auxiliary DNA and gold nanoparticle.The molecule hair fastener probe is the molecule hair fastener of fluorescein (FAM) label.The AuNPs with the method for reduction of sodium citrate gold chloride by being synthesized.Detection method are as follows: 0.01 g gold chloride is dissolved in 100mL ultrapure water, is heated at reflux and boils；2.5 mL1 wt% sodium citrate solutions are quickly added with stirring, are cooled to room temperature after reacting 30 min, AuNPs is prepared；By mercuric nitrate and H1, H2, H3(30-300 nmol/L) and auxiliary DNA(20-500 nmol/L) Tris-HCl buffer solution mix, at room temperature react 1-5 h, then be added AuNPs reaction 5 min；It is detected respectively with ultraviolet-visible spectrophotometer and fluorimeter.This dual signal biosensor preparation based on AuNPs and catalysis hair fastener assembling without enzyme amplifying technique proposed by the present invention is cheap, detection sensitivity is high, overcomes the narrow disadvantage of the applicability of single detection pattern. |
| priorityDate | 2016-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 48.