http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106359083-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-008 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
| filingDate | 2016-08-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106359083-B |
| titleOfInvention | A kind of cultural method for reducing Transgenic Tobacco seedling pollution rate and promoting its Rapid Rooting |
| abstract | The present invention relates to Transgenic Tobacco method and technology fields, specifically disclose a kind of cultural method for reducing Transgenic Tobacco seedling pollution rate and promoting its Rapid Rooting.Using tobacco leaf as receptor, by Agrobacterium tumefaciens mediated method, is screened using Selective agar medium and obtain resistance test material, promote its Rapid Rooting seedling；Tobacco leaf and Agrobacterium infect processing 20 minutes in the Agrobacterium suspension infected liquid containing 200 μm of ol/L acetosyringones, can smoothly remove the Agrobacterium tumefaciems adhered on callus, without influencing receptor growth；Base is co-cultured using the liquid of additional 1g/L cephalosporin and 200mg/L dithiothreitol (DTT) after co-culturing 3 days to clean, can significantly reduce receptor pollution rate, improves transformation efficiency；Selective differentiation culture medium containing 200mg/L cephalosporin and 50mg/LKan can quickly generate callus and a large amount of bud points；The adventitious bud of 1-3cm long is transferred to root media to be conducive to quickly form root system. |
| priorityDate | 2016-08-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 51.