http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106318965-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-865 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 |
| filingDate | 2015-06-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106318965-B |
| titleOfInvention | The integration method of artificial semi-synthetic chromosome and the microorganism for containing completely synthesis chromosome |
| abstract | Microorganism the invention discloses artificial semi-synthetic chromosomal integration method and containing complete synthesis chromosome, this method comprises: (1) provides the first artificial semi-synthetic chromosome and the second artificial semi-synthetic chromosome respectively, wherein, described first artificial semi-synthetic chromosome and the second artificial semi-synthetic chromosome include synthesis chromosomal section and wild type part, have between described first artificial semi-synthetic chromosome and the second artificial semi-synthetic chromosome and integrates homologous region, described first artificial semi-synthetic chromosome and the second artificial semi-synthetic chromosome carry predetermined restriction enzyme siteļ¼(2) the described first artificial semi-synthetic chromosome and the second artificial semi-synthetic chromosome are subjected to homologous recombination in the bacterial strain for expressing predetermined enzyme, to obtain complete synthesis chromosome, wherein, the predetermined enzyme being capable of predetermined restriction enzyme site described in specific recognition.This method can be efficiently used for the integration of two artificial semi-synthetic chromosome. |
| priorityDate | 2015-06-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.