| abstract |
The invention relates to the field of biotechnology and provides a single PCR detection primer pair group aiming to a fungus producing aflatoxin, a detection method, and an application thereof in detection on the fungus producing aflatoxin. In the invention, the single PCR detection primer pair group is composed of primer pairs that aim to a fungus universal molecular marker sequence (internal transcribed spacer, ITS) and three key genes, comprising an aflatoxin production regulating gene (aflR), a sterigmatocystin-o-methoxy transferase gene (omt-1), and versicolorin A dehydrogenase gene (ver-1), in the biochemically synthetic process of the aflatoxin. Through the primer pair group, technical effects of wide coverage range, strong specificity and high sensitivity during the detection of the fungus producing aflatoxin are achieved. The primer pair group is short in detection time, is simple in operation and is low in device requirement, can save large amount of manpower, materials and costs, is suitable for quick detection and is easy to promote in practical production. |