http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106282227-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 |
| filingDate | 2015-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106282227-B |
| titleOfInvention | High-density fermentation method of naked plasmid of recombinant human hepatocyte growth factor |
| abstract | The invention relates to the technical field of biological pharmacy, and particularly provides a high-density fermentation method of a naked plasmid of a recombinant human hepatocyte growth factor. The high-density fermentation method of the naked plasmid of the recombinant human hepatocyte growth factor comprises the following steps: 1) culturing working seeds; 2) inoculating into a fermentation tank for fermentation. The fermentation method can realize high expression of the naked plasmid of the recombinant human hepatocyte growth factor in 50L scale fermentation, the final thallus concentration OD600 can reach more than 55, and the content of the naked plasmid can reach more than 420mg/L, thereby solving the technical problems that the content of the naked plasmid of the recombinant human hepatocyte growth factor is low and the yield can not reach the scale requirement, and laying a solid foundation for the industrialization of the naked plasmid of the recombinant human hepatocyte growth factor. |
| priorityDate | 2015-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 53.