http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106282217-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2445 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01021 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-42 |
| filingDate | 2016-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106282217-B |
| titleOfInvention | A kind of expression vector of β-glucosidase mutant protein, expression engineering bacteria and expression method |
| abstract | The invention discloses an expression vector of a β-glucosidase mutant protein, which is constructed by the following methods: (1) cloning the DNA sequence of the β-glucosidase mutant protein; (2) cloning the DNA of a photosensitive control unit Sequence; (3) the DNase fragment of the β-glucosidase mutant protein obtained by cutting and the DNase fragment of the photosensitive control unit are connected to the pET22b (+) carrier, to obtain the β-glucosidase mutation expression vector for somatic proteins. In the present invention, a light-inducible expression vector is constructed by inserting a photosensitive control unit, and the expression vector can induce expression of the β-glucosidase mutant protein by means of illumination. Compared with the traditional induction expression method, the illumination induction method has the advantages of expressing It has the characteristics of high amount and no exogenous additives. In addition, the expression intensity of the protein by the light induction method can be controlled by the light intensity without affecting the subsequent purification steps. |
| priorityDate | 2016-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 53.