http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106282212-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K31-7105 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K31-7105 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K48-00 |
| filingDate | 2016-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106282212-B |
| titleOfInvention | Preparation and application of triple artificial miRNA (microribonucleic acid) for inhibiting VEGFRs (vascular endothelial growth factors) |
| abstract | The invention discloses a carrier for inhibiting VEGFRs by triple artificial miRNA and a construction method and application thereof. The carrier of the triple artificial miRNA for inhibiting VEGFRs is in pcDNA TM 6.2-GW/miR carrier constructs artificial miRNAs respectively aiming at 3 target genes VEGFR1, VEGFR2 and VEGFR3, wherein the 3 artificial miRNAs are connected to a psliencer4.1 skeleton carrier in a series mode to form a triple amiRNA carrier, and play a role in simultaneously silencing the three genes of VEGFR1, VEGFR2 and VEGFR 3. The triple amiRNA disclosed by the invention can be used for reducing the proliferation of pancreatic cancer cells, promoting the increase of apoptosis and reducing the migration and invasion capacity of the cells; in a nude mouse transplanted pancreatic cancer model, the triple amiRNA-VEGFRs remarkably reduce the growth of tumors, have synergistic effect with chemotherapeutic drugs and are related to the inhibition of epithelial-mesenchymal transition; and has no effect on pancreatic morphology, peripheral blood insulin and blood glucose levels. Therefore, the triple artificial miRNA for inhibiting the VEGFRs has good application in preparing malignant tumors with high expression of the VEGFRs such as pancreatic cancer. |
| priorityDate | 2016-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 253.