http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106282205-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-06 |
filingDate | 2015-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-106282205-B |
titleOfInvention | High-specific-activity L-glutamic acid oxidase gene multi-site mutant and preparation method and application thereof |
abstract | A high specific activity L-glutamic acid oxidase gene multi-site mutant, its preparation method and application, the amino acid sequence of its coded protein is shown in SEQ ID No. 52. The high-specific-activity L-glutamic acid oxidase gene multi-site mutant is prepared by screening a high-activity L-glutamic acid oxidase gene by adopting a molecular in-vitro recombination technology, and preparing the high-specific-activity L-glutamic acid oxidase gene multi-site mutant by vector construction, enzyme activity screening, a multi-site mutation technology and the like, wherein the multi-site mutant contains 9 different mutation sites on the same gene, so that the oxidation specificity and the specific activity of the multi-site mutant to L-glutamic acid are greatly improved, and the high-specific-activity L-glutamic acid oxidase gene multi-site mutant can be applied to. |
priorityDate | 2015-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 413.