http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106258955-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-008 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
| filingDate | 2016-08-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106258955-B |
| titleOfInvention | A method of induction Siraitia grosvenorii albuminous cell seedling |
| abstract | The invention discloses a kind of methods for inducing Siraitia grosvenorii albuminous cell seedling, comprising the following steps: (1) fruit screens；(2) step (1) is obtained into Lo Han Guo fruit and carries out outer surface disinfection treatment；(3) obtain Siraitia grosvenorii seed and carry out sterilization treatment: (4) obtain albuminous cell: the kind shell for the seed for being crossed sterilization treatment with sterile knife is cut, and is taken out the blastocyte in seed, then taken out the albuminous cell in blastocyte with aseptic nipper, is obtained albuminous cell；(5) culture medium is prepared, the group of culture medium is divided into MS+(0.5 ~ 1.5) 6-BA+(0.05 ~ 0.5 mg/L) mg/L NAA.(6) inoculated and cultured: albuminous cell obtained in step (4), which is inoculated into the culture medium that step (5) is prepared, to be cultivated can induce seedling.The present invention can obtain a large amount of aseptic seedling by tissue culture technique, and reproduction speed is fast, plant structural integrity, solve the problem of Siraitia grosvenorii percentage of seedgermination is low, and Explants In Tissue Culture induces seedling microbiological contamination power high survival rate. |
| priorityDate | 2016-08-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 23.