| abstract |
The invention discloses a method for rapid constant temperature detection of Cronobacter sakazakii, a primer set and a kit. The method is as follows: extracting genomic DNA from the sample to be tested; using the genomic DNA as a template and using a primer set capable of amplifying the specific sequence of Cronobacter sakazakii as primers, performing constant temperature amplification under an enzyme reaction system Reaction; determine whether there is Cronobacter sakazakii in the sample to be tested by judging whether the reaction result is positive. The detection method of the invention has high sensitivity and high specificity, short detection time, simple result judgment, convenient operation, low cost and wide application prospect. |