http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106244625-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8205 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-82 |
| filingDate | 2016-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106244625-B |
| titleOfInvention | A kind of tobacco seed genetic transforming method of mediated by agriculture bacillus rapidly and efficiently |
| abstract | The present invention discloses a kind of tobacco seed genetic transforming method of mediated by agriculture bacillus rapidly and efficiently.The key step of this method includes: that mature tobacco seed is used as genetic transformation material after moderately grinding, adjustment Agrobacterium bacterial concentration is to appropriate value for infecting acceptor material, successively after co-cultivation, sowing, GUS histochemical stain and PCR amplification, transgenic tobacco plant is identified.The method of the present invention is without carrying out disinfection, sterilizing and tissue cultures, and most of operating process is without aseptically carrying out, avoid the disadvantages of cumbersome, time-consuming due to sterile and tissue culture operation bring, it is a kind of simple, quick, at low cost, repeated preferable genetic transforming method, has great importance and be worth for quickly formulating transgene tobacco new germ plasm. |
| priorityDate | 2016-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.