http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106244624-B

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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65
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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00
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http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82
filingDate 2016-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2019-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2019-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-106244624-B
titleOfInvention Plasmid system and its application for the building of plant polygene expression vector
abstract The invention discloses a kind of plasmid systems and its application for the building of plant polygene expression vector.By being transformed to e. coli plasmid vector pUC18 and double-core Agrobacterium Ti plasmid pBI121, recombinant plasmid pKAFCR0 and pKAFCR100 are formd.PKAFCR0 has the most common 35S promoter of gene expression in plants and NOS terminator, and at its upstream, intermediate and downstream introduce multiple clone's restriction enzyme sites, can be connected in many ways among the 35S and NOS of pKAFCR0 using the target gene fragment that the methods of PCR clone obtainsï¼›PKAFCR100 has kanamycins NPT II tolerance gene and sGFP Green fluorescent protein fusion vector and in-between polyclone enzyme enzyme site.Two plasmids of pKAFCR0 and pKAFCR100 match, and construct the single or multiple expression vectors of plant with can be convenient.
priorityDate 2016-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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