| abstract |
The present invention relates to echinococcosis multilocularis animal model technical field, it it is the construction method of the echinococcosis multilocularis animal model of a kind of Echinococcus multilocularis THPV approach infected liver, carry out as follows: mouse anesthesia is fixed, mouse web portion is sterilized, open abdomen and expose hepatic portal vein, THPV percutaneous puncture-inoculation Echinococcus multilocularis, pressing point of puncture obtains the echinococcosis multilocularis animal model of Echinococcus multilocularis THPV approach infected liver until closing normal raising after abdomen, percutaneous puncture-inoculation the mice after closing abdomen revive after Zhi Xue.The echinococcosis multilocularis animal model that the construction method of the echinococcosis multilocularis animal model of Echinococcus multilocularis THPV approach infected liver of the present invention obtains has access expansion route of infection and the high advantage of infection rate concurrently；Thus for studying host's liver damage mechanisms caused by different time and dosage mouse infection rate, the mechanism of immunologic tolerance, Alveolar echincoccus, and provide a good animal model for the medicine that screening treatment echinococcosis multilocularis is new. |