http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106234047-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01G7-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01G7-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01G7-00 |
| filingDate | 2016-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106234047-B |
| titleOfInvention | A kind of explant selection method and application for the conversion of mediated by agriculture bacillus soybean cotyledon node |
| abstract | The invention discloses a kind of explant selection methods and application for the conversion of mediated by agriculture bacillus soybean cotyledon node.The present invention provides a kind of methods for preparing the explant for soybean cotyledon node conversion, include the following steps: 1) to plant soya seeds, chooses the seed in following 6 kinds sprouting forms: Y state, YL state, HG state, HG+CG state, CG state, G state；2) seed in 6 kinds of sprouting forms is subjected to gus gene transient transfection and dyeing, the statistics transfection poststaining rate derived from 6 kinds of sprouting form seeds respectively；The significantly high corresponding seed in certain sprouting form of wherein rate of dyeing is chosen as candidate seed；3) cotyledonary node for intercepting the candidate seed, obtains the explant converted for soybean cotyledon node.The experiment proves that existing method to soybean cotyledon node explant only since a certain sprout time, will cause explant selection not comprehensively.The explant that method of the invention is chosen is comprehensive, helps to improve the efficiency of Genetic Transformation of Soybean. |
| priorityDate | 2016-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 30.