| abstract |
The present disclosure provides novel maize, tomato and soybean U6, U3, U2, U5 and 7SL snRNA promoters for CRISPR/Cas-mediated targeted genetic modification in plants. The present disclosure also provides methods of using U6, U3, U2, U5, and 7SL promoters to drive expression of sgRNA polynucleotides that function in CRISPR/Cas systems for targeted genetic modification in plants. The present disclosure also provides genome modification methods for inserting blunt-ended DNA fragments into genomic cleavage sites. |