http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106222141-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-515 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-2302 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-2315 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-599 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0634 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0646 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0783 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-078 |
| filingDate | 2016-10-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106222141-B |
| titleOfInvention | NK cell culture fluids and cell culture processes |
| abstract | The application belongs to cell technology field, and in particular to cell culture fluid and cell culture processes and its application.Cell culture fluid provided by the invention, including：Include the cell factors such as OKT3, CD16, IL 2, IL 15 and 4 1BBL in culture solution A and culture solution B, the culture solution A, includes the cell factors such as IL 2, IL 15 and 4 1BBL in the culture solution B.The present invention also provides a kind of cell culture processes, including：Cell inoculation is cultivated in culture solution A, supplements the culture solution A every other day；Culture continues to cultivate to supplement culture solution B at the 9th day, supplements the culture solution B every other day.Present invention optimizes combination of cytokines and culture process flow, not only shorten cultivation cycle, and making culture only needs 14 21 days, and the NK cell purities that culture obtains reach 70% or more；For the method for the present invention without being purified to NK cells, step is easy, and operability is strong. |
| priorityDate | 2016-10-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 44.