| abstract |
A kind of method quantitative determining albumin content in biological sample and application, the method is by specific binding to ACDM and albumin and form fluorophore conjugate, this conjugate can be excited under conditions of pH value 1-9 and produce stable fluorescence signal, its fluorescent value intensity is directly proportional to albuminous concentration, can extrapolate albuminous content in biological sample according to the fluorescent value detected.The method can be used for measuring albuminous absolute content in the multiple biological sample such as blood plasma, urine, cell culture fluid, the most also have highly sensitive, accuracy is high, environment resistant interference performance strong, be prone to the advantages such as high throughput testing. |