http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106148417-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-865 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P23-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 |
| filingDate | 2015-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-04-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-04-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106148417-B |
| titleOfInvention | Design and construction method of standardized biological element and application thereof |
| abstract | The invention discloses a design and construction method of a standardized biological element and application thereof. The invention discloses a construction method of a standardized biological element, which comprises the following steps: (1) constructing a target gene vector group, a promoter vector group and a terminator vector group. The invention designs and constructs a series of standardized biological elements based on a yeast system, and utilizes II type restriction endonuclease to assemble the standardized biological elements into an extra-large-length exogenous DNA fragment, wherein the exogenous DNA fragment is a target product synthesis path consisting of a plurality of genes, and the fragment is integrated on a specific site of a yeast chromosome through yeast homologous recombination to perform stable expression, so that heterologous synthesis in a target product yeast can be realized, and the optimal expression of the target product can be realized by optimizing promoters of all genes, thereby having unique advantages and important significance in the optimization process of target product synthesis. |
| priorityDate | 2015-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 38.