http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106148310-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y305-05001 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
filingDate | 2016-08-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2019-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2019-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-106148310-B |
titleOfInvention | A kind of nitrilase mutants and its application in niacin preparation |
abstract | The invention discloses a kind of nitrilase mutants and its application in niacin preparation, the mutant is the 168th phenylalanine of amino acid sequence shown in sequence SEQ ID NO.2 to be sported to valine and 192 mutant serines are phenylalanine.Using the recombination engineering of the encoding gene containing nitrilase mutants in the wet thallus obtained after inducing expression as enzyme source, using nicotinonitrile as reaction substrate, reaction system is constituted as reaction medium using the PBS buffer solution of pH=7.0, the water-bath at 40 DEG C, reaction terminates to obtain niacin. |
priorityDate | 2016-08-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 284.