| abstract |
The present invention provides one to be used for efficiently separating the mitochondrial method of Stem Cell Activity, comprises the steps: 1) mescenchymal stem cell detection surface markers is identified, identify to bone, cartilage and Adipose Differentiation ability；2) trypsinization, centrifugal collecting cell；Resuspended cleaning centrifuges；3) preparing mitochondria extract: A liquid is lysate, C is to terminate reactant liquor.4) A liquid resuspended cleaned after cell precipitation carry out vortex, standing, be repeated several times；C liquid is added to stop cracking, centrifugal that white precipitate remains the 5th step operation；Supernatant centrifuges to obtain white precipitate again；5) precipitation centrifuging first in the 4th step, repeats the operation of the 4th step；6) white precipitate of the 4th step and the 5th step is put together, centrifuge to obtain white precipitate, use immediately or 80 degree of preservations.The inventive method separate obtain mitochondria activity good, purity is high, it is adaptable to mescenchymal stem cell separate obtain mitochondria, and easy and simple to handle, do not need grind. |