http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106119344-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6818 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-682 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2016-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106119344-B |
| titleOfInvention | Nano probe for detecting DNA by combining fluorescence intensity and fluorescence polarization |
| abstract | The invention discloses a nano probe for detecting DNA by combining fluorescence intensity and fluorescence polarization, belonging to the technical field of nucleic acid detection. The nano-probe comprises fluorescent protein, a capture probe, a report probe and a nano-gold particle; wherein, the fluorescent protein is used as a donor to be connected with the capture probe, and the nano-gold particles are used as an acceptor to be connected with the report probe; the capture probe and the report probe form an energy transfer donor acceptor pair through complementary pairing of partial bases; the capture probe is perfectly complementary to the target DNA. The nanoprobe combines a high-sensitivity fluorescence intensity parameter and a fluorescence polarization parameter, and realizes non-amplification type DNA high-sensitivity detection; meanwhile, the ratio of fluorescence intensity/fluorescence polarization is introduced by utilizing the opposite change trend of fluorescence intensity and fluorescence polarization in the process of formation and disappearance of resonance fluorescence energy transfer, and the detection signal of the DNA is amplified. |
| priorityDate | 2016-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 30.