http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106119269-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2830-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-101 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2016-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106119269-B |
| titleOfInvention | Method for preparing linear single-stranded DNA in escherichia coli |
| abstract | The invention discloses a method for preparing linear single-stranded DNA, which comprises the following steps: constructing a Cas expression element capable of expressing the Cas nickase and integrating the Cas expression element into a plasmid; constructing a sgRNA gene transcription expression element compatible with the Cas expression element in an escherichia coli cell, and constructing a plasmid to obtain a sgRNA expression plasmid; the Cas nickase expression plasmid and the sgRNA expression plasmid are jointly transformed into an escherichia coli competent cell; culturing escherichia coli to logarithmic growth phase, inducing Cas nickase gene expression and suicide sgRNA expression, and allowing the Cas nickase to cooperate with the sgRNA to self-shear double-stranded DNA of sgRNA expression plasmid and form single-stranded break to generate linear single-stranded DNA; extracting linear single-stranded DNA. |
| priorityDate | 2016-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 74.