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filingDate 2016-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2019-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2019-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-106117291-B
titleOfInvention A kind of fluorescence nucleosides and its preparation method and application for cell imaging
abstract The fluorescence nucleosides and its preparation method and application that the invention discloses a kind of for cell imaging.Such nucleosides fluorescent dye has lower background fluorescence in the few cell compartment of rna content and tissue, there is stronger fluorescence signal in the high cell compartment of rna content and tissue, and when the viscosity of cell changes, fluorescence intensity and service life also change therewith.Since granulophilocyte only contains RNA, so such fluorescent molecule can filter out granulophilocyte from haemocyte, and multi-modal imaging is carried out to it.This kind of compound has certain solubility in water, while having good water oil amphipathic, can pass through cell membrane;And also there is biggish effective two photon absorption cross section.In addition, this kind of compound of the invention also has lower bio-toxicity, photobleaching and high photostability, and the spectral region of itself of its spectral region and biological sample has sufficiently large difference.
priorityDate 2016-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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