http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106110290-B

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filingDate 2016-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2019-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2019-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-106110290-B
titleOfInvention A kind of preparation method of animal testis extract
abstract The present invention relates to polypeptide extractive technique field, in particular to a kind of preparation method of animal testis extract.The preparation method of the animal testis extract includes: to be homogenized animal testis, enzymolysis and extraction, obtains enzymatic hydrolysis crude extract and filter residue;Enzymatic hydrolysis crude extract obtains ultrafiltrate through ultrafiltration, and ultrafiltrate removes macromolecule impurity through Ago-Gel Hydrogen Binding Adsorption chromatographic process again, obtains enzymolysis and extraction refined liquid;Filter residue extracts concentration using ethyl alcohol, obtains concentration alcohol extract;Enzymolysis and extraction refined liquid is mixed with concentration alcohol extract, is freeze-dried, obtains animal testis extract.The method that testis extraction and preparation technique of the present invention is combined using toolenzyme extraction, ultrafiltration, agarose Hydrogen Binding Adsorption chromatographic separation and purification, it is enriched with micromolecule polypeptide class active material, filter residue alcohol extracting concentration and recovery testosterone not soluble in water, reduces the loss of active constituent.Its method is easy, and safely and effectively, product bioactivity is good.
priorityDate 2016-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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