http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106106147-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-008 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
| filingDate | 2016-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106106147-B |
| titleOfInvention | A kind of renovation process of lotus unmature subleaf somatic embryo development ways |
| abstract | The invention discloses a kind of renovation process of lotus unmature subleaf somatic embryo development ways, it includes：(1) explant of the unmature subleaf as tissue cultures in lotus after fertilization zygotic embryo is chosen, is carried out disinfection to explant and cleaning treatment；(2) explant is put into embryo callus subculture induction with being cultivated in proliferated culture medium, induces callus and carry out Multiplying culture；(3) callus lines are transferred to embryoid to occur in culture medium, development forms embryoid；(4) embryoid of generation is moved into embryo germination culture medium and is sprouted, development forms cotyledon shape embryo；(5) the cotyledon shape embryo of sprouting is moved into seedling culture in the culture medium of seedling containing lotus, then transplanted into natural environment.The explant physical efficiency of the present invention differentiates one piece of embryo callus subculture group, therefrom can efficiently break up the somatic embryo of more than 1000 and finally sprout, and the transgenosis of lotus can be promoted to develop and provide substantial amounts of seedling for the growth condition of lotus. |
| priorityDate | 2016-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.