http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106103742-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-158 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6886 |
| filingDate | 2015-01-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106103742-B |
| titleOfInvention | A method and reagent for enriching circulating tumor DNA |
| abstract | The invention discloses a method and reagents for enriching circulating tumor DNA. The method comprises the following steps: mixing and oscillating an aqueous phase and an oil phase to form an emulsion PCR reaction system and performing emulsion PCR amplification, wherein the aqueous phase contains Peripheral blood plasma DNA, forward and reverse primers, dNTPs, PCR buffer and DNA polymerase as template DNA, the two ends of peripheral blood plasma DNA are connected with adapter sequences, and the forward and reverse primers are complementary to the adapter sequences at both ends; emulsion After PCR amplification, the water phase and the oil phase are separated to obtain the PCR amplification product of the water phase; the circulating tumor DNA in the PCR amplification product of the water phase is captured by using a probe sequence that specifically binds to the circulating tumor DNA. The method of the present invention can effectively capture ctDNA in peripheral blood plasma, and perform single-molecule high-fidelity ultra-micro-parallel amplification to provide sufficient amount of ctDNA for subsequent sequencing and detection. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110240999-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110240999-B |
| priorityDate | 2015-01-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 146.