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classificationCPCAdditional http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-025
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filingDate 2016-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2018-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2018-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-106093303-B
titleOfInvention The screening technique of tabacum sterol mutant
abstract The invention discloses a kind of screening technique of tabacum sterol mutant, comprise the following steps:(1) tobacco seed, is handled with ethylmethane sulfonate, obtains M1 for seed;(2), M1 is planted for seed, single plant harvests after maturation, obtains M2 for seed;(3), the 1/2MS culture mediums containing voriconazole of gradient concentration are prepared;(4), using the 1/2MS culture mediums containing voriconazole of step (3) gradient concentration, tobacco seed is cultivated, according to the growth and development of tobacco seed, obtains the gradient concentration of the corresponding 1/2MS culture mediums containing voriconazole of tobacco seed growth and development;(5), using the 1/2MS culture mediums containing voriconazole of step (4) gradient concentration, to cultivate M2 for seed, the state of growing according to M2 for seed, screens the minimum and highest mutant of tabacum sterol content.Screening technique of the present invention is easy to operate, cost is low, efficient, is applicable to mass mutation body and colony's single plant screening.
priorityDate 2016-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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