| abstract |
The present invention discloses the iPSCs of a kind of non-viral iPSCs abductive approach and its inducing composition, kit and its acquisition.Specifically, which is building up in plasmid episomal the following steps are included: 1) will express the DNA sequence dna for reprogramming the factor POU5F1, SOX2, GLIS1, KLF4, MYCL and hsa-miR-302s, obtains recombinant plasmid；2) recombinant plasmid for obtaining step 1) imports in the body cell of people, carries out Fiber differentiation, obtains iPSCs.For this method in the case where being added without the high risk reprogramming factor such as c-MYC, SV40-LT, TP53 inhibiting factor, reprogramming combinations of factors safe to use, the clinic for reducing iPSCs is applicable in risk；This method applicability with higher. |