http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106086009-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-87 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-11 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-87 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-13 |
| filingDate | 2016-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106086009-B |
| titleOfInvention | Method for producing all-pig-derived monoclonal antibody by using single B cell PCR technology |
| abstract | The invention discloses a method for producing a full-pig-derived monoclonal antibody by using a single B cell PCR technology, belonging to the technical field of biology. The invention designs a plurality of sets of primers by utilizing the existing pig antibody gene library, and amplifies the antibody variable region gene from a single B lymphocyte by utilizing nested PCR (polymerase chain reaction), thereby screening out the full-pig-derived monoclonal antibody with neutralizing antibody activity. The invention establishes a method for producing the all-pig-source monoclonal antibody by utilizing a high-throughput single B cell technology for the first time, has the advantages of high efficiency, all-pig source, good gene diversity and the like compared with the traditional antibody preparation technology, and provides a new technical means for researching the immune response of the antibody of the pig and separating the therapeutic antibody. |
| priorityDate | 2016-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.