http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106053829-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-70571 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-186 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-94 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-18 |
| filingDate | 2016-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106053829-B |
| titleOfInvention | It is a kind of based on measure p38MAPK protein expressions to detect the method for zebra fish M3 receptor proteins |
| abstract | It is a kind of based on measure p38MAPK protein expressions to detect the method for zebra fish M3 receptor proteins, comprise the following steps：(1) preparation of zebra fish brain tissue protein sample；(2) sds gel electrophoresis；(3) transferring film；(4) close；(5) primary antibody, secondary antibody are incubated；(6) chromogenic reaction；(7) M3 acceptor result of variations is calculated.No specific aim antibody is limited by instant invention overcomes the detection of traditional zebra fish M3 acceptors and can not use the defect of protein immunoblotting technology, it is proposed first using p38MAPK phosphorylation levels so as to determine zebra fish M3 receptor protein changes of contents, this method high sensitivity, stability are good, do not need complex device；While simple and quick, specific height of the invention, cost are low, the present invention studies the inherent mechanism of action of cholinergic system in terms of molecule, in conjunction with the research in terms of zebra fish Behavioral change, can finally realize the real-time online assessment to water quality with monitoring. |
| priorityDate | 2016-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 44.