http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106053403-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6486 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2016-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-05-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-05-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106053403-B |
| titleOfInvention | A kind of protein quantification detection method |
| abstract | The present invention relates to field of biotechnology, the detection methods complicated and time consumption such as ELISA takes reagent in quick real-time detection to solve the disadvantage that current albumen, the disadvantages of non-specific adsorption and modifying interface of electrochemical detection method, the invention proposes a kind of protein quantification detection methods, by testing protein sample, adjust probe, expand template, nickase, archaeal dna polymerase, buffer, dNTPs, magnesium chloride, mixed solution is made in SYBR Green I fluorescent dye, it carries out amplification reaction under isothermal conditions, quantitative relationship will be established between testing protein concentration and the appearance time of isothermal duplication curve, realize the Real_time quantitative detection of albumen.This method has simple, specificity high, versatile and can be with advantages such as Real_time quantitative detections, and sub- nanomole grade can be reached by detecting limit, be can satisfy general Protein Detection and required. |
| priorityDate | 2016-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 39.