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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-705
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http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851
filingDate 2016-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2020-05-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2020-05-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-106048094-B
titleOfInvention Dual real-time fluorescent quantitative PCR (polymerase chain reaction) detection kit, primers and probe for porcine pseudorabies wild strains and gene-deleted strains
abstract The invention relates to a dual real-time fluorescent quantitative PCR detection kit for detecting gB genes and gE genes of a porcine pseudorabies wild strain and a gene deletion strain, a special primer and a TaqMan probe thereof. The invention establishes a dual real-time fluorescent quantitative PCR method aiming at the gB gene and the gE gene, can rapidly identify the pseudorabies wild strain (containing the gB gene and the gE gene simultaneously) and the gene deletion strain (containing only the gB gene), and can accurately quantify the virus copy number. The kit and the detection method have the advantages of simple and convenient operation, strong specificity and high sensitivity, and can play a role in the detection of the porcine pseudorabies virus, the identification of the strain and the production of the vaccine.
priorityDate 2016-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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