http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106048065-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6888 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6888 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2016-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106048065-B |
| titleOfInvention | Mandarin sturgeon environment DNA detects PCR amplification primer and its detection method and application |
| abstract | The invention belongs to field of biotechnology, and in particular to mandarin sturgeon environment DNA detects PCR amplification primer and its detection method and application.The PCR amplification primer ASCB1F sequence are as follows: 5 '-ACAATGCCACCCTTAC-3 ', ASCB1R sequence are as follows: 5 '-TGTCTGCGTCTGAGTTT-3 '.The DNA molecular marker site of mandarin sturgeon species is located at mandarin sturgeon mitochondria CYTB gene, DNA fragmentation length is 138bp, DNA sequence dna is as shown in SEQ ID NO.1, environment DNA amplification is carried out using the PCR amplification primer, amplified production is compared with DNA molecular marker site bit sequence, and the non-matching of guiding region 100% is mandarin sturgeon species.The present invention realizes the identification to mandarin sturgeon species using environment DNA detection, without acquiring fish body sample, can avoid the damage to fish body. |
| priorityDate | 2016-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 50.