Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4f50ebe93b17d5ab34200decdb446e33 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A23V2002-00 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A23L3-3571 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2462 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H15-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01017 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-6436 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-72 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A23L3-3571 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H15-04 |
filingDate |
2016-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ece5144a22e458fcd07afa5c62a25601 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b04e1570d61d1699b11efc5c0e65c2b2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5433be03823e0678b881bed72650255d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_06899f024ec2d3248dd90857bad91cd6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3a6e1b100324523ac8339785f0ab35e8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e70a3b611f8abd1575db42b62400ac5b |
publicationDate |
2016-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CN-106047845-A |
titleOfInvention |
A kind of lysozyme covalently modified by short aliphatic chain acyl sophorolipid derivatives and its modification method and application |
abstract |
The invention discloses a lysozyme covalently modified by using a carboxylic acid type short aliphatic chain acyl sophorolipid derivative and its modification method and application. The modification method comprises the following steps: (1) making the sophorolipid derivative Dissolve the active ester in DMSO, then add the NaHCO 3 aqueous solution of lysozyme, carry out the condensation reaction under stirring conditions, and obtain the reaction mixture after the reaction; (2) using deionized water and phosphate buffer to dialyze the buffer system obtained in step (1) sequentially, and obtain the modified lysozyme through centrifugal separation of the obtained dialyzate. The modified lysozyme can not only effectively inhibit the activity of Gram-positive bacteria, but also significantly inhibit the activity of Gram-negative bacteria, thereby expanding the antibacterial spectrum of natural lysozyme, and can be used as a multifunctional food preservation agent. |
priorityDate |
2016-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |