http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106011191-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-101 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y113-12002 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y305-0105 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0069 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-005 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
filingDate | 2016-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2019-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2019-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-106011191-B |
titleOfInvention | A kind of method of Whole Cell Biocatalysis production 5- aminovaleric acid |
abstract | The invention discloses a kind of using expressing L-lysine 2- monooxygenase DavB and δ-pentanamide hydrolase DavA Bacillus coli cells as catalyst, the method that biocatalysis produces 5- aminovaleric acid, is to expressing DavBA E.coli BL21(DE3 low temperature induction expression) is carried out, collecting bacterium then as catalyst in buffer system, makes cell OD in system 600nm For 5-90, lysine concentration 0-400g/L, and under the auxiliary of metal ion and surfactant, method that whole-cell catalytic produces 5- aminovaleric acid.It is long that the present invention solves the fermentation method production cycle, metabolite is complicated, the substrate transformation rate is low, product separation and Extraction is difficult, and the problem that energy consumption is high, it also solves enzyme law catalysis cascade catalytic process and is not easy the deficiency realized, improve catalytic efficiency, cumbersome enzyme purification process is eliminated simultaneously, production cost is lower. |
priorityDate | 2016-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 49.