http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106011128-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-03 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-03 |
| filingDate | 2016-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106011128-B |
| titleOfInvention | Joint transfer method of saccharopolyspora erythraea industrial production strain |
| abstract | The invention discloses a conjugal transfer method of saccharopolyspora erythraea industrial production strain. The method comprises pre-treating mature Saccharopolyspora erythraea spores, and placing into Mg-containing container 2+ In the TSB liquid culture medium, after germination in a static water bath, washing and suspending to obtain recipient bacteria; the donor bacterium and the acceptor bacterium spore are mixed and cultured in a coating 2CMY solid joint transfer plate culture medium to grow the joint transfer seed. The method is convenient to operate, simple and feasible, and the conjugation transfer efficiency of saccharopolyspora erythraea can reach 2 × 10 ‑5 The method can well overcome the situation that the genetic operation of the prior saccharopolyspora erythraea industrial production strain is difficult, and provides convenient conditions for the directed genetic modification and the gene function research of the saccharopolyspora erythraea industrial strain. |
| priorityDate | 2015-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 24.