http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105985944-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2015-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105985944-B |
| titleOfInvention | A kind of new method of intracellular site specific covalent labeled RNA |
| abstract | The present invention relates to it is a kind of in vitro or the new method of intracellular site specific covalent labeled RNA.This method by introducing tRNA in vitro or into the cell lle2 - agmatidine synzyme (abbreviation Tias, nucleotide sequence is as shown in SEQ ID NO:1, and amino acid sequence is as shown in SEQ ID NO:2) and the ancient green-ball bacterium tRNA of flashing lle2 (SEQ ID NO:3) or its derivatized nucleotide sequence, make AftRNA in Tias specific recognition transcript profile lle2 Particular sequence, promote the small molecule containing azide or alkynyl functionality and to contain tRNA lle2 Sequence is generated between the target RNA of label and locus specificity combines, and then specific marker and the imaging of target RNA are realized by the chemical reaction of fluorescent dye and functional group.The invention further relates to the kits of locus specificity covalent labeling target RNA in the cell, and it includes tRNA lle2 - agmatidine synzyme and tRNA lle2 ‑3‑5。 |
| priorityDate | 2015-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 64.