| abstract |
An expression enhancer is provided comprising a CPMV 5'UTR nucleotide sequence consisting of X nucleotides (CMPVX), wherein X=160 in SEQ ID NO: 1, 155, 150 or 114, or consist of nucleotide sequences comprising about 80% to 100% sequence similarity to CMPVX, wherein X=160, 155, 150 or 114. The expression enhancer may also comprise a stuffer sequence (CMPVX+, where X=160, 155, 150 or 114 of SEQ ID NO: 1) fused to the 3' end of the 5'UTR nucleotide sequence. The stuffer sequence may comprise one or more plant kozak sequences. Plants comprising the expression enhancers and methods of utilizing the expression enhancers are also described. |