| abstract |
An expression enhancer is provided comprising a CPMV 5'UTR nucleotide sequence consisting of X nucleotides (CMPVX), where X=160 of SEQ ID NO:1, 155, 150, or 114, or consist of a nucleotide sequence comprising about 80% to 100% sequence similarity to CMPVX, wherein X=160, 155, 150, or 114. The expression enhancer may also comprise a stuffer sequence fused to the 3' end of the 5'UTR nucleotide sequence (CMPVX+, where X=160, 155, 150 or 114 in SEQ ID NO: 1). The stuffer sequence may comprise one or more plant kozak sequences. Also described are plants comprising the expression enhancers and methods of using the expression enhancers. |