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filingDate 2016-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2019-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2019-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-105969667-B
titleOfInvention A kind of strain of scenedesmus obliquus algae and its cultural method and application
abstract A kind of isolation and purification method of scenedesmus obliquus algae strain SoHNCJ2, is uniformly coated on the BG11 culture medium flat plate of 1.5-2% agar after the water sample of acquisition is diluted, places and carry out continuous illumination culture in the incubator;Fall behind to grow single algae on plate, is fallen in toothpick picking list algae and draw short-term culture on BG11 plate;The algae that gained is drawn to short-term culture again draws the separation and degerming that Z-shaped carries out algae with transfer needle, and algae then is further purified with the method that dilution applies plate;Picking list algae fall draw short-term and picking short-term algae fall draw Z-line alternately 2~4 times after obtain pure single algae and fall.N the or P concentration of Fresh Watcr Blue Algae culture medium BG11 is reduced to original 1/4 or increases to original three times, that is, uses the BG11 culture medium of 1/4 N or 3 times of N, P concentration by the cultural method of scenedesmus obliquus algae strain SoHNCJ2 a kind of.Scenedesmus obliquus of the invention can be grown continuously and healthily under the conditions of the source N of low concentration, and frustule can effectively accumulate a large amount of greases, can carry out turning esterification production biodiesel.
priorityDate 2016-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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