| abstract |
The invention discloses nanogels of a kind of autofluorescence and the preparation method and application thereof.The present invention prepares nanogel by combination reverse phase nanoprecipitation method and light-operated " tetrazolium-alkene " click chemistry method, " tetrazolium-alkene " the click chemistry cross-linking method used have many advantages, such as specificity it is strong, efficiently quickly, without catalyst, this can effectively maintain the bioactivity of the drug contained and protein, have a good application prospect in the fields such as drug controlled release carrier.The cross-linking method of nanogel disclosed by the invention has very strong selectivity, with the drug contained, especially pharmaceutical grade protein and cell does not react, the effect of capable of keeping drug, protein and cell well, complete, controllable release is realized, so as to the excellent slow-released carrier as protein and drug；Reach affected area, nanogel slow releasing pharmaceutical, the problem of reaching effective therapeutic effect, not will cause drug waste in the prior art. |