http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105950593-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-101 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-2105 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-57 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2016-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105950593-B |
| titleOfInvention | A kind of the pronuclear recombination expression and preparation method of lysyl peptide chain endopeptidase |
| abstract | The invention discloses a kind of expression of the pronuclear recombination of lysyl peptide chain endopeptidase and preparation methods.The preparation method includes: the encoding gene that the recombination lysyl peptide chain endopeptidase that amino acid sequence is sequence 2 is imported into recipient E. coli cell, obtains recombinant Bacillus coli cells；Inducing expression is carried out to it, is collected and is crushed recombinant Bacillus coli cells, obtain occlusion body；Then it is successively denaturalized, renaturation, activation, purifying, obtains recombination lysyl peptide chain endopeptidase.Using recombination Lys-C protease prepared by preparation method of the invention there is high enzyme activity and higher urea to be resistant to.Preparation method of the invention, which has the advantages that enzyme, specificity is good, animal origin-free is viral etc. has, and produces simple, at low cost with preparation process, can be applied to bio-pharmaceuticals and proteomics research. |
| priorityDate | 2016-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 100.