http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105950541-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2740-15021 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2740-15043 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-867 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
| filingDate | 2016-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105950541-B |
| titleOfInvention | A kind of construction method of hFGF21 gene knockout human liver cell strain |
| abstract | The invention discloses a kind of construction methods of hFGF21 gene knockout human liver cell strain, this method is by design hFGF21 target site primer and synthesizes sgRNA oligonucleotides chain-ordering, then recombinant slow virus plasmid is constructed, it obtains recombinant slow virus and transfects cell, stablize and knock out hFGF21 gene, obtains the human liver cell strain of hFGF21 gene knockout；The method of the present invention is simple, and sgRNA targeting is good, knocks out high-efficient. |
| priorityDate | 2016-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 181.