http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105936907-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8205 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-415 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8216 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-29 |
filingDate | 2016-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2017-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2017-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-105936907-B |
titleOfInvention | A kind of breeding method for reducing rice grain cadmium content |
abstract | The invention discloses a kind of breeding method for reducing rice grain cadmium content, including:The OsLCT1 extrons of cloning rice acceptor material;Using CRISPR/Cas9 systems, target sequence is selected according to exon sequence, builds pCRISPR/Cas9 recombinant vectors;Transgenic seedling will be obtained in pCRISPR/Cas9 recombinant vector Introduced into Rice callus;Screening transgenic positive plant;Obtain mutant plant;Mutant plant is subjected to breeding, afunction mutant of the separation without transgene component in Progeny plants.The present invention knocks out rice Os LCT1 using CRISPR/Cas9 technologies orientation, thoroughly inactivation cadmium transport protein OsLCT1, orientation is bred as without transgene component, Cd concentration of brown rice significantly reduces, rice material of the comprehensive agronomy character without significant variation, have it is safe efficient, save the advantages such as time, cost be low. |
priorityDate | 2016-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.