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http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-062
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filingDate 2016-04-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2018-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2018-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-105929062-B
titleOfInvention LC-MS method for detection of kaempferol in Luo Han Guo tea
abstract The invention provides a method for detecting kaempferol in Luo Han Guo tea by liquid-mass series, aiming to provide a method for detecting kaempferol in Luo Han Guo tea by liquid-mass series with high sensitivity, qualitative and quantitative accuracy; the method is: 1) taking This product, accurately weighed, finely ground, ultrasonically treated, centrifuged to remove the lower precipitate, and dried; 2) In a microwave reactor, add chlorinated 1-alkyl-3-methylimidazole and methanol, and treat at 60°C for 10 minutes Obtain the extract; 3) Concentrate the extract to obtain a concentrate, add macroporous resin to the concentrate, mix and stir evenly, apply AB-8 resin after drying, wash with water until colorless, then rinse with ethanol solution, collect and wash Deliquification, washing, concentration and drying to obtain a dry powder; 4) Weigh 0.5 g of the dry powder in step 3), add 5 ml of 10% phosphoric acid solution, heat and reflux in a water bath for 30 minutes, let cool, quantitatively transfer to a 25 ml measuring bottle, and use methanol Dilute to the scale, filter the membrane, and take the filtrate for detection; it belongs to the technical field of chemical detection.
priorityDate 2016-04-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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