http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105928927-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-76 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-76 |
filingDate | 2016-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2019-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2019-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-105928927-B |
titleOfInvention | The method that electrogenerated chemiluminescence imaging method quickly analyzes unicellular interior ingredient |
abstract | The present invention provides the method utilization that a kind of electrogenerated chemiluminescence imaging method quickly analyzes unicellular interior ingredient, luminol electrogenerated chemiluminescence is applied to the detection of unicellular interior biomolecule (such as glucose) for the first time.In the detection process, individual cells are positioned directly at micro- hole electrode of individual cells size;After the mixed solution containing luminol, TritonX-100 and glucose oxidase is added, TritonX-100 can destroy cell membrane, discharge glucose oxidase reaction of the intracellular glucose into micro- hole and in hole and generate hydrogen peroxide;Hydrogen peroxide is reacted with luminol generates optical signal under positive potential.This neoteric electrochemical luminescence measuring method would be possible to be applied to quickly to analyze more intracellular molecules in unicellular, to illustrate intercellular heterogeneity. |
priorityDate | 2016-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 45.