http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105924525-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-28 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-14 |
filingDate | 2016-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-105924525-B |
titleOfInvention | Preparation method and application of porcine TLR4 polyclonal antibody |
abstract | The invention discloses a preparation method of a porcine TLR4 polyclonal antibody, which is characterized in that a recombinant TLR4 protein (antigen) and Freund's adjuvant are emulsified and then immunized to an animal to obtain polyclonal antibody serum; wherein, the recombinant TLR4 protein has an amino acid sequence of SEQ ID No.1 of a sequence table. Tests show that the antibody prepared by the invention has good reactivity and specificity, and the TLR4 gene is a member of a Toll-like receptor family of an important pattern recognition receptor aiming at the innate immune response and plays an important role in resisting bacterial and viral infectious diseases, so the invention can be applied to the detection and related research of the porcine TLR4 protein, lays a good foundation for the research of the interaction mechanism of the porcine bacterial and viral infectious diseases and the TLR4 and the escape mechanism of virus immunity, and provides a new target for the development of novel vaccines and medicaments. Meanwhile, the prokaryotic expression vector constructed by the invention has high efficiency of expressing recombinant protein, and the separation and purification method is simple and easy to operate. |
priorityDate | 2016-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 103.