http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105918131-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-001 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
filingDate | 2016-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2018-02-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2018-02-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-105918131-B |
titleOfInvention | A kind of Radix Angelicae Sinensis callus inducing medium |
abstract | The invention provides a kind of Radix Angelicae Sinensis callus inducing medium, it is characterised in that the proportioning of the culture medium is B 5 The D+1.2 3.0mg/L NAA of+11.0+0.6 0.9mg/L of 17.0ug/ml hybrid giant napier amino acid extract solutions 2,4.Present invention also offers a kind of method of Radix Angelicae Sinensis callus induction, the described method comprises the following steps:The Radix Angelicae Sinensis tender tissue position of robust growth is chosen, successively with 90% ethanol disinfection 12s, 0.1%HgCl after cleaning 2 Sterilize 8min, after aseptic water washing 3 times, be cut into 4mm segments, be inoculated in Radix Angelicae Sinensis callus inducing medium described in claim 1, the 18d of incubated at room temperature 15, illumination 8h/d under 2200 2400lx intensities of illumination.Beneficial effects of the present invention are that induction time is short;Inductivity is high, applied widely, suitable popularization and application;It can cultivate to obtain high polyoses content cell. |
priorityDate | 2016-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 27.