http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105911167-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-027 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 |
| filingDate | 2016-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105911167-B |
| titleOfInvention | The assay method of methacrylaldehyde DNA adduct in a kind of saliva |
| abstract | The assay method of methacrylaldehyde DNA adduct in a kind of saliva, that is α Acr dG and γ Acr dG assay method, including collecting saliva using the saliva collection tubes of OG 500, DNA extractions are carried out to saliva, DNA solution carries out enzyme hydrolysis and sequentially adds stable isotope internal standard and DNA hydrolases.Hydrolyzate collects eluent, nitrogen is blown to dry at room temperature, is redissolved in 100 μ L 30% methanol aqueous solution, is introduced LC MS/MS network analyses, can accurately detect α Acr dG and γ Acr dG contents level through the small column solid phase extractions of Strata X.The present invention can reduce caused error in sample pretreatment process using stable isotope as uantitative analytical thing, and tandem mass spectrometry preferably improves the selectivity, accuracy and sensitivity of method.By chromatographic column and the selection and optimization of elution requirement, the separating degree of object is preferably improved. |
| priorityDate | 2016-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 65.